Anti-mutagenic Property of Fish Oil from Tamban (Sardinella lemuru Family Clupeidae) in Female ICR Mice

Asian Journal of Pharmaceutical and Health Sciences,2016,6,3,1522-1526.
Published:September 2016
Type:Research Article
Author(s) affiliations:

Nadia Czarina Mae Cortuna1, Liza Marie De Guzman2, Reylene Paula De Lara3, Jennelyn Delos Santos4, Efryl Geronimo5, Julius Ceazar Mendoza5, Charlene Keilah Ong6, Catherine Reyes7, Mylene Andal MS Pharm2, Cecilia Santiago 2*

1Department of Health Region IV-B Office QMMC Compound, Project 4, Quezon City, Philippines. 

2Centro Escolar University, School of Pharmacy, Manila, Philippines.

3Mercury Drug Corporation, Acacia Lane, Mandaluyong City, Philippines

4Christ’s Commission Fellowship, Frontera Verde, Ortigas Avenue corner C5 Road, Pasig City, Philippines.

5Pointwest Innovations Corporation, UP Ayala Land Technohub, Quezon City, Philippines. 

6Romblon Provincial Hospital Liwanag, Odiongan, Romblon, Philippines

7Centro Escolar University, Department of Pharmacy, Makati City, Philippines


This study aimed to determine the anti-mutagenic property of fish oil from Tamban (Sardinella lemuru Family Clupeidae) in Mitomycin C – induced female ICR mice. Initially, twelve mice were selected for acute oral toxicity testing. The resulted LD50 dose was 2000 mg/kg b.w. Twenty-five mice were then randomly divided into five groups: group I – positive control; group II- negative control and groups III, IV and V as experimental groups. Group I was given 5 mg/kg b.w. of Mitomycin C, group II 5 mL/ kg b.w. of normal saline solution, lastly groups III, IV and V received ¼ LD50 (500 mg/kg b.w), ½ LD50 (1000 mg/ kg b.w.), ¾ LD50 (1500 mg/ kg b.w.) of fish oil for seven days, respectively. After the last dose, Mitomycin C was administered intra peritoneal. After 24 hours, bone marrows were obtained and Micronucleus Test was performed. About two thousand erythrocytes were counted for the presence of Micronucleated Polychromatic Erythrocytes (MNPCE) and Polychromatic Erythrocytes (PCE). The results showed that 500 mg per kilogram body weight of Tamban fish oil could exhibit anti-mutagenic property comparable to the negative control. It also possessed the greatest MNPCE inhibition. However, 1000 mg per kilogram body weight and 1500 mg kilogram body weight showed no significant difference with the positive control and significant difference with the negative control. The results proved that the fish oil extract possessed potential anti-mutagenic property against Mitomycin C-induced female ICR mice.

Average Micronucleated Polychromatic Erythrocytes (MNPCE) per Test Group