Nephroprotective Potential of Trichosanthes dioica ROXB. Leaves Extract Against Gentamicin Induced Nephropathy in Albino Rats

Asian Journal of Pharmaceutical and Health Sciences,2015,5,3,1300-1305.
Published:August 2015
Type:Research Article
Author(s) affiliations:

Ramesh Kumar Gupta1,2,*, Sudhansu Ranjan Swain1, Padala Narasimha Murthy2,  Jagannath Sahoo3, Preet Verma4, Chandana Venkateswara Rao4, Amresh Gupta5

1Sherwood college of of Pharmacy, Barabanki -225001, Uttar Pradesh, India.
2Royal College of Pharmacy and Health Sciences, Berhampur -760002, Orissa, India.
3Shri Ram Murti Smarak College of Engineering and Technology-Bareilly-243202, Uttar Pradesh, India.
4Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
5Goel Institute of Pharmacy and Sciences, Lucknow-226024 Uttar Pradesh, India.


The purpose of this study is to evaluate nephroprotective potential of Trichosanthes dioica leaves extract against Gentamicin (GM) induced nephrotoxicity and renal dysfunction. Twenty-four Wistar rats were divided into four groups (n=6). Control rats that received normal saline (i.p.) and 0.5% carboxymethyl cellulose (p.o.) per day for 8 days. Nephrotoxicity was induced in rats by intraperitoneally administration of GM (100 mg/kg/day for 8 days) and were treated with TLE (200 and 400 mg/kg/day (p.o.) for 8 days). Plasma and urine urea and creatinine, kidney weight, urine output, blood urea nitrogen, urinary sodium and potassium level, renal enzymatic and non-enzymatic antioxidants and lipid peroxidation was evaluated along with histopathological investigation in various experimental groups of rats. It was observed that the GM treatment induced significant elevation (p < 0.001) in plasma and urine urea, creatinine, kidney weight, + + blood urea nitrogen, plasma Na and K level, renal lipid peroxidation along with significant decrement (p< 0.001) in urine output, renal enzymatic and non-enzymatic antioxidants. TLE 200 and 400 mg/kg treatment to GM treated rats recorded significant decrement (up to p < 0.001) in plasma and urine urea + + and creatinine, urinary Na and K level, renal lipid peroxidation along with significant increment (up to p < 0.001) in renal enzymatic and non-enzymatic antioxidants. Histological observations of kidney tissues too correlated with the biochemical observations. These finding powerfully supports that Trichosanthes dioica leaves extract acts in the kidney as a potent scavenger of free radicals to prevent the toxic effects of GM both in the biochemical and histopathological parameters.

Histological study of kidney tissue in control and experimental groups of rats