Bacterial Cellulose Production by Acetobacter xylinum and Saccharomyces Cerevisiae in Green Tea Leaves and Fruits Juice Medium

Asian Journal of Pharmaceutical and Health Sciences,2013,3,2,690-698.
Published:May 2013
Type:Research Articles
Author(s) affiliations:

DC Moretti Vieira*, BTG Senna, M Ishii, TC Vessoni Penna

Department of Pharmaceutical Technology, Pharmaceutical Science College, University of São Paulo, Brazil.


The biomembrane (bacterial cellulose) was developed by Gluconacetobacter xylinus ATTC 23769, associated to Saccharomyces cerevisiae, for 7-10 days cultivation, from residual fruit-vegetable juices added to green tea broth, was studied and hydroalcoholic Calendula officinalis extract (1:1) was incorporated into biomembrane. Cultures were grown 1200 mL flasks with 80% v/v broth (Green Tea, Orange, Papaya, Wine, Collagen and Beet broths) and 20% v/v inoculum, incubated for 7-10 days at 28±2°C under static conditions, pH initial 3.5 – 4.0. The biomembrane thickness, diameter and weight were measured. The DSC, FTIR, BET analyses were performed and Water and Marigold absorption capacity were calculated.The highest bacterial cellulose yield (6.1±0.5 g cellulose/day) was obtained in green tea medium with 40% v/v papaya juice and also 10% v/v orange juice. Moreover, the addition of 1.2% w/v collagen to the green tea medium increased by 1.5 times the biomembrane weight. The developed biomembrane is composed by cellulose (confirmed by infrared spectroscopy (FTIR) at λmax = 1644 cm-1), with a total surface of 2.07 m2/g, an average porous size of 158 Å (BET), strength and elasticity between 0.09-0.5 MPa, (similar to Young’s model for indentation) and thermal stability up to 137°C (DSC). The biomembrane absorption capacity for water and hydroalcoholic Calendula officinalis extract (1:1), was six and five times higher than its dry weight, respectively. The Marigold extract was incorporated into the biomembrane due to its suitable topical properties, such as antioxidant, anti-inflammatory and wound healing.

Biomembrane weight (g); productivity (g cellulose/day); conversion factor (g cellulose/ g sugar) and thickness (mm) in the green tea medium (green tea), orange medium (orange), wine (wine medium); papaya (papaya medium); beet 8% (beet medium with 8% v/v beet juice) and beet 15% (beet medium with 15% v/v beet juice), 10 days of cultivation, initial pH = 3.5.